Proteomic and transcriptomic profiles of human urothelial cancer cells with histone deacetylase 5 overexpression.
Ananda Ayyappan Jaguva VasudevanMichèle Janine HoffmannGereon PoschmannPatrick PetzschConstanze WiekKai StühlerKarl KöhrerWolfgang A SchulzGuenter NiegischPublished in: Scientific data (2022)
Urothelial carcinoma (UC) of the urinary bladder is a prevalent cancer worldwide. Because histone deacetylases (HDACs) are important factors in cancer, targeting these epigenetic regulators is considered an attractive strategy to develop novel anticancer drugs. Whereas HDAC1 and HDAC2 promote UC, HDAC5 is often downregulated and only weakly expressed in UC cell lines, suggesting a tumor-suppressive function. We studied the effect of stable lentiviral-mediated HDAC5 overexpression in four UC cell lines with different phenotypes (RT112, VM-Cub-1, SW1710, and UM-UC-3, each with vector controls). In particular, comprehensive proteomics and RNA-seq transcriptomics analyses were performed on the four cell line pairs, which are described here. For comparison, the immortalized benign urothelial cell line HBLAK was included. These datasets will be a useful resource for researchers studying UC, and especially the influence of HDAC5 on epithelial-mesenchymal transition (EMT). Moreover, these data will inform studies on HDAC5 as a less studied member of the HDAC family in other cell types and diseases, especially fibrosis.
Keyphrases
- lymph node metastasis
- histone deacetylase
- papillary thyroid
- rna seq
- single cell
- squamous cell carcinoma
- epithelial mesenchymal transition
- cell proliferation
- endothelial cells
- dna methylation
- transcription factor
- gene expression
- mass spectrometry
- stem cells
- mesenchymal stem cells
- high grade
- bone marrow
- squamous cell
- electronic health record
- young adults
- deep learning
- case control
- drug induced