Miktoarm Star-polypept(o)ide-Based Polyion Complex Micelles for the Delivery of Large Nucleic Acids.

Miktoarm star polymers exhibit a captivating range of physicochemical properties, setting them apart from their linear counterparts. This study devised a synthetic pathway to synthesize cationic miktoarm stars utilizing polypept(o)ides (PeptoMiktoStars), comprising 3 or 6 polysarcosine (pSar) arms (AB 3×100 , AB 6×50 , overall 300) for shielding and a cross-linkable poly( S -ethylsulfonyl-l-homocysteine) (pHcy(SO 2 Et) 20 ) block and a poly(l-lysine) ((pLys) 20 ) block for nucleic acid complexation. Precise control over the DP n and narrow molecular weight distributions ( D̵ ≈ 1.2) were achieved for both structures. Both PeptoMiktoStars efficiently complexed mRNA and pDNA into polyion complex micelles (PICMs). AB 6 -PICMs provided modest (mRNA) to high (pDNA) stability against glutathione and heparin sulfate (HS), while even cross-linked AB 3 -PICMs were susceptible to HS. All PICMs delivered pDNA and mRNA into D1 cells (over 80%) and Jurkat T cells (over 50%) in vitro. Despite payload- and cell-dependency, AB 3 showed overall higher transfection efficiency, while AB 6 demonstrated better shielding and enhanced stability.