Oxygen-Induced Retinopathy from Recurrent Intermittent Hypoxia Is Not Dependent on Resolution with Room Air or Oxygen, in Neonatal Rats.
Kay D BeharryCharles L CaiJacqueline SkeltonFaisal SiddiquiChristina D'AgrosaJohanna CaloGloria B ValenciaJacob V ArandaPublished in: International journal of molecular sciences (2018)
Preterm infants often experience intermittent hypoxia (IH) with resolution in room air (RA) or hyperoxia (Hx) between events. Hypoxia is a major inducer of vascular endothelial growth factor, which plays a key role in normal and aberrant retinal angiogenesis. This study tested the hypothesis that neonatal IH which resolved with RA is less injurious to the immature retina than IH resolved by Hx between events. Newborn rats were exposed to: (1) Hx (50% O₂) with brief hypoxia (12% O₂); (2) RA with 12% O₂; (3) Hx with RA; (4) Hx only; or (5) RA only, from P0 to P14. Pups were examined at P14 or placed in RA until P21. Retinal vascular and astrocyte integrity; retinal layer thickness; ocular and systemic biomarkers of angiogenesis; and somatic growth were determined at P14 and P21. All IH paradigms resulted in significant retinal vascular defects, disturbances in retinal astrocyte template, retinal thickening, and photoreceptor damage concurrent with elevations in angiogenesis biomarkers. These data suggest that the susceptibility of the immature retina to changes in oxygen render no differences in the outcomes between RA or O₂ resolution. Interventions and initiatives to curtail O₂ variations should remain a high priority to prevent severe retinopathy.
Keyphrases
- diabetic retinopathy
- optical coherence tomography
- optic nerve
- vascular endothelial growth factor
- endothelial cells
- rheumatoid arthritis
- disease activity
- preterm infants
- high glucose
- interstitial lung disease
- systemic lupus erythematosus
- single molecule
- physical activity
- gene expression
- type diabetes
- systemic sclerosis
- dna methylation
- low birth weight
- electronic health record
- copy number
- deep learning
- data analysis