Antibacterial and wound-healing potential of PLGA/spidroin nanoparticles: a study on earthworms as a human skin model.
Fatma El-Zahraa A Abd El-AzizHelal F HettaBasma N AbdelhamidNoura H Abd EllahPublished in: Nanomedicine (London, England) (2022)
Aim: The essential protein element of spider silk 'spidroin' was used to assess its impact on the wound-healing process. Methods: Spidroin nanoparticles (NPs) were prepared using poly(lactic-co-glycolic acid) polymer (PLGA/spidroin NPs) at different weight ratios (5:1, 10:1, 15:1) and were in vitro characterized. The optimized NPs were tested in vitro for release and antibacterial activity. To assess wound-healing effects, NPs were topically applied on surgically induced injuries in Allolobophora caliginosa earthworms as a robust human skin model. Results: Optimized NPs (173 ± 3 nm) revealed considerable antibacterial effect against Staphylococcus aureus and Escherichia coli . After 4 days of NPs application on wounds, macroscopical and histological examinations revealed a significant reduction in wound and re-epithelialization times. Conclusion: PLGA/spidroin NPs may represent a promising option for wound repair.
Keyphrases
- wound healing
- oxide nanoparticles
- escherichia coli
- drug delivery
- staphylococcus aureus
- body mass index
- silver nanoparticles
- oxidative stress
- risk assessment
- photodynamic therapy
- biofilm formation
- pseudomonas aeruginosa
- climate change
- endothelial cells
- bone regeneration
- binding protein
- multidrug resistant
- essential oil
- surgical site infection