Micromorphological investigation of leaf epidermis and seeds of Vitaceae from Pakistan using light microscopy and scanning electron microscopy.
null LubnaMuhammad ZafarMushtaq AhmadShabnum ShaheenShazia SultanaSaeed Ur RehmanHafiza AminaPublished in: Microscopy research and technique (2018)
This present study is the first report on the detailed foliar epidermal anatomy and micromorphology of seeds of five species of Vitaceae from Pakistan using light microscopy (LM) and scanning electron microscopy (SEM). The studied species occur at various localities particularly higher altitude. Qualitative and quantitative leaf micromorphological characters investigated here are related to shape and size of epidermal cells, anticlinal wall pattern, stomatal pore, guard cell, stomatal complex, subsidiary cells, and trichomes. Variations in size and shape were observed in the studied species. Irregular, polygonal and elongated epidermal cells with straight or undulate margins, stomata anomocytic type, and trichomes nonglandular, unicellular to multicellular in all the studied plants. Seed micromorphological qualitative characters studied are shape of margins, integument cell, apical notch, chalaza, anticlinal wall thickness, and quantitative characters are number of seeds per berry, length, and width of seed, length to width ratio. The detailed distribution and microscopic characteristics investigated of family Vitaceae has a distinguished role in identification at genus and species level and may provide evidence in the determination of taxonomic rank of the family in the phylogenetic tree. This study possesses potential for plant taxonomists to further evaluate the species for phytochemical studies and physiology. RESEARCH HIGHLIGHTS: Light microscopy and scanning electron microscopy was used for the micromorphological investigation of family Vitaceae Qualitative and quantitative characteristics were studied Variation in microscopic features of leaf epidermis and seeds Identification of species based on micromorphological characters.
Keyphrases
- solid state
- electron microscopy
- high resolution
- induced apoptosis
- cell cycle arrest
- single cell
- single molecule
- optical coherence tomography
- genetic diversity
- systematic review
- high speed
- endoplasmic reticulum stress
- cell proliferation
- tertiary care
- cell therapy
- stem cells
- cell death
- oxidative stress
- risk assessment
- label free
- bioinformatics analysis
- pi k akt