Chemical constituents from Cordia myxa L. (Boraginaceae) and their antibacterial activity.

Chemical investigation of Cordia myxa L. (Boraginaceae) resulted in the isolation of the following ten known compounds: 1-naphthaleneacetic-5-carboxy-1,2,3,4,4a,7,8,8a-octahydro-1,2,4a-trimethyl-[1 S -(1 α ,2 β , 4a,8a α )]-acid ( 1 ), hexacosanoate-1-glyceryl ( 2 ), 3 β -urs-12,20(30)-diene-27,28-dioic acid ( 3 ), 3 β -D-glucopyranosylurs-12,20(30)-diene-27,28-dioic acid ( 4 ), stigmasterol ( 5 ), stigmasterol-3- O - β -D-glucopyranoside ( 6 ), oleanolic-acid ( 7 ), 3- O -acetyl-oleanolic acid ( 8 ), betulin ( 9 ) and spinasterol-3 β - O -D-glucopyranoside ( 10 ). The isolated compounds were characterised by using spectroscopic methods, 1D and 2D NMR, mass spectroscopy (ESI-MS) and by comparison with the literature data. To the best of our knowledge, compounds 1 , 3 , 4 , 8 and 10 were isolated for the first time from the Cordia genus. This result improves the chemotaxonomy knowledge of the Cordia genus. The antibacterial activities were performed by the Muller-Hinton agar diffusion method. The antibacterial activities were studied on Salmonella typhi , Staphylococcus aureus , Vibrio cholerae , Pseudomonas aeruginosa and Escherichia coli ATCC 25922. Compounds 8 and 9 , at 20.0 mg/mL resulted to be effective antimicrobial against E. coli , V. cholerae and P. aeruginosa.