Effects of Different Doses of Multienzyme Supplementation on Growth Performance, Duodenal pH and Morphology, and Carcass Traits in Broilers Fed Diets with an Increasing Reduction in Energy.

This study evaluated the effects of supplementing different doses of a multienzyme (KZP) consisting of carbohydrases and a protease on growth performance, duodenal pH and morphology, and carcass traits in broilers fed diets with increasing reductions in energy. One thousand two hundred one-day-old broiler chicks were allocated to five dietary treatments with eight replicates of 30 birds each: a positive control diet formulated to meet Arbor Acres' nutritional requirements (PC); a negative control diet reformulated to 80 kcal/kg less than the apparent metabolizable energy (AME) of the PC (NC1); a negative control diet reformulated to 120 kcal/kg less than the AME of the PC (NC2); an NC1 diet supplemented with 300 g/t of KZP (NC1 + KZP300); and an NC2 supplemented with 500 g/t of KZP (NC2 + KZP500). Growth performance was measured throughout the study. At 35 days, 10 birds per treatment were randomly selected and euthanized for a carcass trait evaluation, and samples of the duodenum were collected for morphological examination and pH level determination. The final average body weight and feed conversion ratio were better ( p < 0.05) for the broilers in the NC1 + KZP300 group compared to those in NC1, NC2 and NC2 + KZP500 groups and were similar to those of the PC birds ( p > 0.05). Birds from the NC1 + KZP500 group showed a better ( p < 0.05) final body weight and feed efficiency compared to the NC1 and NC2 groups. The villus height was greater ( p < 0.05) for the PC and NC1 + KZP300 groups compared to the rest of the treatments. The crypt depth was longer ( p < 0.05) for the NC1 and NC2 groups compared to the NC1 + KZP300 group. The supplementation of KZP to both the NC1 and NC2 diets reduced ( p < 0.05) the abdominal fat %. This study demonstrates that supplementing energy-reduced diets with KZP improved performance in broiler chickens.