Doxorubicin-Conjugated Iron Oxide Nanoparticles Synthesized by Laser Pyrolysis: In Vitro Study on Human Breast Cancer Cells.
Iulia Ioana LunguSimona NistorescuMădălina Andreea BadeaAndreea-Mihaela PetreAna Maria UdreaAna-Maria BaniciClaudiu FleacăEcaterina AndronescuAnca DinischiotuFlorian DumitracheAngela StaicuMihaela BalasPublished in: Polymers (2020)
Even today, breast cancer remains a global public problem, with a high mortality rate among women. Nanoparticle (NP) based systems are developed to enhance drug delivery, reducing the toxic effect of medicine molecules. By using iron oxide nanoparticles for cancer treatment, several advantages were highlighted: the ability to target specific locations derived from their magnetic properties and reduced side effects. The aim of this study was to examine on breast cancer cell line the anticancer potential of γ-Fe2O3 NPs loaded with doxorubicin (DOX) and stabilized with carboxymethylcellulose sodium (CMCNa). The γ-Fe2O3 NPs were synthesized by laser pyrolysis technique and their nanometric size and crystallinity were confirmed by X-ray diffraction and transmission electron microscopy. The loading efficiency was estimated by using absorption and fluorescence spectroscopy. The DOX conjugated//CMCNa coated γ-Fe2O3 NPs proved through the biological studies to have a good anticancer effect through the inhibition of tumoral cell proliferation, disruption of the cellular membrane, induction of cell death and reduced effects on normal breast cells. Our data showed that DOX cytotoxicity increases significantly when conjugated with ɣ-Fe2O3 and ɣ-Fe2O3_CMCNa, a 50% reduction of cancer cell viability was obtained with a concentration around 0.1 µg/mL.
Keyphrases
- drug delivery
- iron oxide nanoparticles
- electron microscopy
- cell death
- cell proliferation
- cancer therapy
- photodynamic therapy
- endothelial cells
- high resolution
- emergency department
- breast cancer cells
- induced apoptosis
- healthcare
- single molecule
- type diabetes
- cell cycle arrest
- computed tomography
- cell cycle
- magnetic resonance
- mental health
- metabolic syndrome
- risk assessment
- pregnant women
- papillary thyroid
- squamous cell carcinoma
- skeletal muscle
- young adults
- endoplasmic reticulum stress
- cardiovascular events
- quantum dots
- mass spectrometry
- artificial intelligence