Establishing a sensitive fluorescence-based quantification method for cyclic nucleotides.

Nadine GruteserViktoria KohlhasSabine BalfanzArne FranzenAnne GüntherAndreas OffenhäusserFrank MüllerViacheslav NikolaevMartin J LohseArnd Baumann

Published in: BMC biotechnology (2020)

We found that determination cAMP in lysates obtained from cell assays or tissue samples by purified Epac1-camps is a robust, fast, and sensitive assay suitable for routine and high throughput analyses.

Keyphrases

high throughput
single cell
single molecule
cell therapy
clinical practice
solid phase extraction
binding protein