Top-Down N-Doped Carbon Quantum Dots for Multiple Purposes: Heavy Metal Detection and Intracellular Fluorescence.
Francesca LimosaniElvira Maria BauerDaniele CecchettiStefano BiagioniViviana OrlandoRoberto PizzoferratoPaolo ProspositoMarilena CarbonePublished in: Nanomaterials (Basel, Switzerland) (2021)
In the present study, we successfully synthesized N-doped carbon quantum dots (N-CQDs) using a top-down approach, i.e., hydroxyl radical opening of fullerene with hydrogen peroxide, in basic ambient using ammonia for two different reaction times. The ensuing characterization via dynamic light scattering, SEM, and IR spectroscopy revealed a size control that was dependent on the reaction time, as well as a more pronounced -NH2 functionalization. The N-CQDs were probed for metal ion detection in aqueous solutions and during bioimaging and displayed a Cr3+ and Cu2+ selectivity shift at a higher degree of -NH2 functionalization, as well as HEK-293 cell nuclei marking.
Keyphrases
- quantum dots
- hydrogen peroxide
- sensitive detection
- energy transfer
- loop mediated isothermal amplification
- heavy metals
- room temperature
- single cell
- nitric oxide
- single molecule
- air pollution
- label free
- real time pcr
- particulate matter
- cell therapy
- risk assessment
- molecular dynamics simulations
- health risk
- stem cells
- metal organic framework
- health risk assessment
- electron transfer
- solid state
- fluorescent probe