Changes in cell wall structure and protein set in Candida maltosa grown on hexadecane.
Anton ZvonarevVasilina FarofonovaEkaterina KulakovskayaTatiana KulakovskayaAndrey MachulinSvyatoslav SokolovVladimir DmitrievPublished in: Folia microbiologica (2020)
The yeast Candida maltosa is a model organism for studying adaptive changes in the structure and function of the cell wall when consuming water-insoluble nutrient sources. The cells of C. maltosa that utilize hydrocarbons contain supramolecular structures, so-called "canals" in the cell wall. Differences in protein profiles of culture liquids and cell wall extracts of C. maltosa grown on glucose and hexadecane were analyzed. Three proteins specific of cells grown on hexadecane were revealed using mass spectrometry: glycosyl hydrolase EPD2 in the culture liquid; a protein belonging to the cytochrome C family in the 0.5 mol/L NaCl extract; and PPIA_CANAL protein known as chaperone, in the 0.1% SDS extract. The possible role of these proteins in cell wall structures responsible for adaptation to hexadecane utilization is discussed.
Keyphrases
- cell wall
- induced apoptosis
- mass spectrometry
- cell cycle arrest
- oxidative stress
- amino acid
- binding protein
- candida albicans
- metabolic syndrome
- cell death
- drinking water
- adipose tissue
- cell proliferation
- blood pressure
- skeletal muscle
- anti inflammatory
- insulin resistance
- high performance liquid chromatography
- quantum dots