Persicaria minor (Huds.) Opiz Prevents In Vitro Atherogenesis by Attenuating Tumor Necrosis Factor-α-Induced Monocyte Adhesion to Human Umbilical Vein Endothelial Cells.
Adila A HamidAmilia AminuddinNur Najmi Mohamad AnuarNur Izzati MansorMohd Faizal AhmadMohammed S M SalehMohd Helmy MokhtarAzizah UgusmanPublished in: Life (Basel, Switzerland) (2022)
Persicaria minor (Huds.) Opiz is an herb with anti-inflammatory, antioxidant, and anti-atherosclerosis effects. Nevertheless, the mechanism underlying its anti-atherosclerosis effect is poorly comprehended. This in vitro study assessed the protective effects of standardized aqueous extract of P. minor leaves (PM) on tumor necrosis factor-α (TNF-α)-induced monocyte adhesion to human umbilical vein endothelial cells (HUVEC), which is one of the pivotal early steps in atherogenesis. The results showed that PM decreased the mRNA and protein expression of cellular adhesion molecules, vascular adhesion molecule-1 and intercellular adhesion molecule-1, resulting in reduced adhesion of monocytes to HUVEC. Additionally, PM inhibited nuclear factor kappaB (NF-κB) activation as indicated by reduced NF-κB p65 levels in TNF-α-induced HUVEC. Overall, PM could prevent in vitro atherogenesis by inhibiting NF-κB activation and adhesion of monocytes to HUVEC. The effects of PM are probably mediated by its bioactive compound, quercetin-3- O -glucuronide. The findings may provide a rationale for the in vivo anti-atherosclerosis effect of PM, and support its potential use in atherosclerosis.
Keyphrases
- particulate matter
- endothelial cells
- high glucose
- nuclear factor
- air pollution
- biofilm formation
- polycyclic aromatic hydrocarbons
- signaling pathway
- oxidative stress
- rheumatoid arthritis
- heavy metals
- anti inflammatory
- cardiovascular disease
- dendritic cells
- diabetic rats
- toll like receptor
- cell adhesion
- cell migration
- water soluble
- pi k akt
- type diabetes
- staphylococcus aureus
- immune response
- ionic liquid
- mouse model
- binding protein
- cystic fibrosis
- candida albicans