New Biosensor for Determination of Neuropilin-1 with Detection by Surface Plasmon Resonance Imaging.
Anna SankiewiczBeata Żelazowska-RutkowskaEwelina GorskaAdam HermanowiczEwa GorodkiewiczPublished in: Sensors (Basel, Switzerland) (2023)
Neuropilin-1 is transmembrane protein with soluble isoforms. It plays a pivotal role in both physiological and pathological processes. NRP-1 is involved in the immune response, formation of neuronal circuits, angiogenesis, survival and migration of cells. The specific SPRI biosensor for the determination of neuropilin-1 was constructed using mouse monoclonal antibody that captures unbound NRP-1 form body fluids. The biosensor exhibits linearity of the analytical signal between 0.01 and 2.5 ng/mL, average precision value 4.7% and recovery between 97% and 104%. The detection limit is 0.011 ng/mL, and the limit of quantification is 0.038 ng/mL. The biosensor was validated by parallel determination of NRP-1 in serum and saliva samples using the ELISA test, with good agreement of the results.
Keyphrases
- tandem mass spectrometry
- solid phase extraction
- label free
- liquid chromatography
- monoclonal antibody
- simultaneous determination
- sensitive detection
- molecularly imprinted
- high resolution
- gold nanoparticles
- loop mediated isothermal amplification
- quantum dots
- immune response
- induced apoptosis
- real time pcr
- wastewater treatment
- cell cycle arrest
- cell death
- endothelial cells
- cell proliferation
- inflammatory response
- fluorescence imaging
- brain injury
- wound healing