Harnessing Efficient ROS Generation in Carbon Dots Derived from Methyl Red for Antimicrobial Photodynamic Therapy.
Raul L FerreiraWalter Muniz JrLouise E A SouzaHector M C NavarroLucas R de MelloValmor R MastelaroTasso O SalesCintya D A E S BarbosaAdriana S RibeiroEmerson Rodrigo da SilvaMelissa F LandellItalo N de OliveiraPublished in: ACS applied bio materials (2023)
The emergence of drug-resistant pathogenic microorganisms has become a public health concern, with demand for strategies to suppress their proliferation in healthcare facilities. The present study investigates the physicochemical and antimicrobial properties of carbon dots (CD-MR) derived from the methyl red azo dye. The morphological and structural analyses reveal that such carbon dots present a significant fraction of graphitic nitrogen in their structures, providing a wide emission range. Based on their low cytotoxicity against mammalian cells and tunable photoluminescence, these carbon dots are applied to bioimaging in vitro living cells. The possibility of using CD-MR to generate reactive oxygen species (ROS) is also analyzed, and a high singlet oxygen quantum efficiency is verified. Moreover, the antimicrobial activity of CD-MR is analyzed against pathogenic microorganisms Staphylococcus aureus , Candida albicans , and Cryptococcus neoformans . Kirby-Bauer susceptibility tests show that carbon dots synthesized from methyl red possess antimicrobial activity upon photoexcitation at 532 nm. The growth inhibition of C. neoformans from CD-MR photosensitization is investigated. Our results show that N-doped carbon dots synthesized from methyl red efficiently generate ROS and possess a strong antimicrobial activity against healthcare-relevant pathogens.
Keyphrases
- oxidative stress
- dna damage
- reactive oxygen species
- staphylococcus aureus
- drug resistant
- living cells
- healthcare
- photodynamic therapy
- candida albicans
- public health
- quantum dots
- contrast enhanced
- fluorescent probe
- magnetic resonance
- biofilm formation
- cell death
- multidrug resistant
- nk cells
- energy transfer
- acinetobacter baumannii
- genome wide
- computed tomography
- signaling pathway
- escherichia coli
- single molecule
- fluorescence imaging
- big data
- health information
- health insurance