Conventional and Real-Time PCR Targeting bla OXA Genes as Reliable Methods for a Rapid Detection of Carbapenem-Resistant Acinetobacter baumannii Clinical Strains.
Dagmara DepkaAgnieszka MikuckaTomasz BogielMateusz RzepkaPatryk ZawadkaEugenia Gospodarek-KomkowskaPublished in: Antibiotics (Basel, Switzerland) (2022)
Multidrug-resistant Acinetobacter baumannii , particularly those producing carbapenemases, are spread worldwide. A reliable detection of carbapenemases is essential to choose the appropriate antimicrobial therapy and, consequently, prevent the dissemination of carbapenem-resistant strains. The aim of this study is to examine the molecular basis of the carbapenem resistance mechanism and estimation of conventional PCR and real-time PCR usefulness for the detection of oxacillinases when compared to phenotypic carbapenemases detection. The following methods were evaluated: the CarbAcineto NP test, Carbapenem Inactivation Method, CPO panels of semiautomated antimicrobial susceptibility testing method on the BD Phoenix™ M50 system, conventional Polymerase Chain Reaction and real-time PCR. The eazyplex ® SuperBug complete A assay was used as the reference method. Among the tested strains, 39 (67.2%) carried the bla OXA-40 gene, while the bla OXA-23 gene was noted amongst 19 (32.8%) isolates. The diagnostic sensitivities of the studied assays were as follows: CarbAcineto NP-65.5%; CIM-100%; CPO-100%; conventional PCR-100%; real-time PCR-100%.
Keyphrases
- real time pcr
- multidrug resistant
- klebsiella pneumoniae
- acinetobacter baumannii
- drug resistant
- gram negative
- escherichia coli
- genome wide
- pseudomonas aeruginosa
- high throughput
- genome wide identification
- copy number
- staphylococcus aureus
- cystic fibrosis
- stem cells
- gene expression
- transcription factor
- genome wide analysis
- bioinformatics analysis