Plasma Proteome Profiling of Coronary Artery Disease Patients: Downregulation of Transthyretin-An Important Event.
null MonuRupsi KharbAnkita SharmaMonu Kumar ChaddarRakesh YadavPrachi AgnihotriAnand KarSagarika BiswasPublished in: Mediators of inflammation (2020)
Coronary artery disease (CAD) is a prevalent chronic inflammatory cardiac disorder. An early diagnosis is likely to help in the prevention and proper management of this disease. As the study of proteomics provides the potential markers for detection of a disease, in the present investigation, attempt has been made to identify disease-associated differential proteins involved in CAD pathogenesis. For this study, a total of 200 selected CAD patients were considered, who were recruited for percutaneous coronary intervention (PCI) treatment. The proteomic analysis was performed using two-dimensional gel electrophoresis (2-DE) and MALDI-TOF MS/MS. Samples were also subjected to Western blot analysis, enzyme-linked immunosorbent assay (ELISA), peripheral blood mononuclear cells isolation immunofluorescence (IF) analysis, analytical screening by fluorescence-activated cell sorting (FACS), and in silico analysis. The representative data were shown as mean ± SD of at least three experiments. A total of 19 proteins were identified. Among them, the most abundant five proteins (serotransferrin, talin-1, alpha-2HS glycoprotein, transthyretin (TTR), fibrinogen-α chain) were found to have altered level in CAD. Serotransferrin, talin-1, alpha-2HS glycoprotein, and transthyretin (TTR) were found to have lower level, whereas fibrinogen-α chain was found to have higher level in CAD plasma compared to healthy, confirmed by Western blot analysis. TTR, an important acute phase transport protein, was validated low level in 200 CAD patients who confirmed to undergo PCI treatment. Further, in silico and in vitro studies of TTR indicated a downexpression of CAD in plasma as compared to the plasma of healthy individuals. Lower level of plasma TTR was determined to be an important risk marker in the atherosclerotic-approved CAD patients. We suggest that the TTR lower level predicts disease severity and hence may serve as an important marker tool for CAD screening. However, further large-scale studies are required to determine the clinical significance of TTR.
Keyphrases
- coronary artery disease
- percutaneous coronary intervention
- coronary artery bypass grafting
- cardiovascular events
- end stage renal disease
- ejection fraction
- acute myocardial infarction
- newly diagnosed
- antiplatelet therapy
- mass spectrometry
- stem cells
- chronic kidney disease
- prognostic factors
- acute coronary syndrome
- high throughput
- aortic stenosis
- peritoneal dialysis
- south africa
- heart failure
- cell therapy
- mesenchymal stem cells
- multiple sclerosis
- signaling pathway
- single cell
- atrial fibrillation
- molecular docking
- quantum dots
- cell proliferation
- single molecule
- combination therapy
- binding protein
- replacement therapy
- cardiovascular disease
- hyaluronic acid