Intramolecular tetrazine-acryloyl cycloaddition: chemistry and applications.
Akil HamsathOren L LederbergQi CuiMeg ShiehYannie LamBrock J BrummettShi XuJerome R RobinsonMing XianPublished in: Chemical science (2022)
An unprecedented intramolecular [4 + 2] tetrazine-olefin cycloaddition with α,β-unsaturated substrates was discovered. The reaction produces unique coumarin-dihydropyridazine heterocycles that exhibited strong fluorescence with large Stokes shifts and excellent photo- and pH-stability. This property can be used for reaction analysis. The rate of cycloaddition was found to be solvent dependent and was determined using experimental data with a kinetic modeling software (COPASI) as well as DFT calculations ( k 1 = 0.64 ± 0.019 s -1 and 4.1 s -1 , respectively). The effects of steric and electronic properties of both the tetrazine and α,β-unsaturated carbonyl on the reaction were studied and followed the known trends characteristic of the intermolecular reaction. Based on these results, we developed a "release-then-click" strategy for the ROS triggered release of methylselenenic acid (MeSeOH) and a fluorescent tracer. This strategy was demonstrated in HeLa cells via fluorescence imaging.
Keyphrases
- fluorescence imaging
- energy transfer
- cell cycle arrest
- electron transfer
- density functional theory
- induced apoptosis
- fluorescent probe
- cell death
- photodynamic therapy
- dna damage
- quantum dots
- oxidative stress
- living cells
- molecular dynamics simulations
- single molecule
- computed tomography
- machine learning
- data analysis
- endoplasmic reticulum stress
- ionic liquid
- monte carlo