Methylation of geometrically constrained lysine analogues by histone lysine methyltransferases.

Abbas H K Al Temimi Paul B WhiteMarcus J M MuldersNicole G A van der LindenRichard H BlaauwAnita WegertFloris P J T Rutjes Jasmin Mecinovic

Published in: Chemical communications (Cambridge, England) (2020)

We report synthesis and enzymatic assays on human histone lysine methyltransferase catalysed methylation of histones that possess lysine and its geometrically constrained analogues containing rigid (E)-alkene (KE), (Z)-alkene (KZ) and alkyne (Kyne) moieties. Methyltransferases G9a and GLP do have a capacity to catalyse methylation in the order K ≫ KE > KZ ∼ Kyne, whereas monomethyltransferase SETD8 catalyses only methylation of K and KE.

Keyphrases

dna methylation
genome wide
endothelial cells
amino acid
molecular docking
gene expression
high throughput
hydrogen peroxide
single cell