Harnessing reaction-based probes to preferentially target pancreatic β-cells and β-like cells.
Sevim KahramanDebasish MannaErcument DiriceBasudeb MajiJonnell SmallBridget K WagnerAmit ChoudharyRohit N KulkarniPublished in: Life science alliance (2021)
Highly sensitive approaches to target insulin-expressing cells would allow more effective imaging, sorting, and analysis of pancreatic β-cells. Here, we introduce the use of a reaction-based probe, diacetylated Zinpyr1 (DA-ZP1), to image pancreatic β-cells and β-like cells derived from human pluripotent stem cells. We harness the high intracellular zinc concentration of β-cells to induce a fluorescence signal in cells after administration of DA-ZP1. Given its specificity and rapid uptake by cells, we used DA-ZP1 to purify live stem cell-derived β-like cells as confirmed by immunostaining analysis. We tested the ability of DA-ZP1 to image transplanted human islet grafts and endogenous mouse pancreatic islets in vivo after its systemic administration into mice. Thus, DA-ZP1 enables purification of insulin-secreting β-like cells for downstream applications, such as functional studies, gene-expression, and cell-cell interaction analyses and can be used to label engrafted human islets and endogenous mouse islets in vivo.
Keyphrases
- induced apoptosis
- cell cycle arrest
- gene expression
- endothelial cells
- endoplasmic reticulum stress
- oxidative stress
- cell death
- signaling pathway
- stem cells
- adipose tissue
- machine learning
- small molecule
- dna methylation
- insulin resistance
- mesenchymal stem cells
- glycemic control
- cell therapy
- big data
- artificial intelligence
- fluorescent probe
- photodynamic therapy