Low Overpotential Amperometric Sensor Using Yb 2 O 3 .CuO@rGO Nanocomposite for Sensitive Detection of Ascorbic Acid in Real Samples.

The ultimate objective of this research work is to design a sensitive and selective electrochemical sensor for the efficient detection of ascorbic acid (AA), a vital antioxidant found in blood serum that may serve as a biomarker for oxidative stress. To achieve this, we utilized a novel Yb 2 O 3 .CuO@rGO nanocomposite (NC) as the active material to modify the glassy carbon working electrode (GCE). The structural properties and morphological characteristics of the Yb 2 O 3 .CuO@rGO NC were investigated using various techniques to ensure their suitability for the sensor. The resulting sensor electrode was able to detect a broad range of AA concentrations (0.5-1571 µM) in neutral phosphate buffer solution, with a high sensitivity of 0.4341 µAµM -1 cm -2 and a reasonable detection limit of 0.062 µM. The sensor's great sensitivity and selectivity allowed it to accurately determine the levels of AA in human blood serum and commercial vitamin C tablets. It demonstrated high levels of reproducibility, repeatability, and stability, making it a reliable and robust sensor for the measurement of AA at low overpotential. Overall, the Yb 2 O 3 .CuO@rGO/GCE sensor showed great potential in detecting AA from real samples.