Cypripedium subtropicum embryo development and cytokinin requirements for asymbiotic germination.
Holger PernerRong ZhouWenqing PernerHong JiangYung-I LeePublished in: Botanical studies (2022)
This study provides a reproducible procedure for culturing immature seeds of C. subtropicum based on a defined time schedule of seed development. In addition, the cytokinin 2iP was shown to improve germination and protocorm survival. This study provides a scientific basis for seedling establishment through asymbiotic seed culture for further reintroduction efforts.
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