Alkaline Phosphatase Immobilization on New Chitosan Membranes with Mg2+ for Biomedical Applications.
Gratiela Teodora TihanRoxana Gabriela ZgârianElena BerteanuDaniela IonitaGeorgeta ToteaCatalin IordachelRodica TatiaMariana ProdanaIoana DemetrescuPublished in: Marine drugs (2018)
In this paper, we present the fabrication and characterization of new chitosan-based membranes while using a new biotechnology for immobilizing alkaline phosphatase (ALP). This technology involved metal ions incorporation to develop new biopolymeric supports. The chemical structure and morphological characteristics of proposed membranes were evaluated by infrared spectroscopy (FT-IR) and the scanning electron microscopy technique (SEM). The inductively coupled plasma mass spectrometry (ICP-MS) evidenced the metal ion release in time. Moreover, the effect of Mg2+ on the enzymatic activity and the antibacterial investigations while using Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria, hemolysis, and biocompatibility behavior were studied. Immobilizing ALP into the chitosan membranes composition followed by the incorporation of Mg2+ led to polymeric supports with enhanced cellular viability when comparing to chitosan-based membranes without Mg2+. The results obtained evidenced promising performance in biomedical applications for the new biopolymeric supports that are based on chitosan, ALP, and metal ions.
Keyphrases
- drug delivery
- gram negative
- mass spectrometry
- wound healing
- electron microscopy
- escherichia coli
- hyaluronic acid
- multidrug resistant
- staphylococcus aureus
- cancer therapy
- high resolution
- liquid chromatography
- ms ms
- multiple sclerosis
- high performance liquid chromatography
- capillary electrophoresis
- biofilm formation
- hydrogen peroxide
- klebsiella pneumoniae
- pseudomonas aeruginosa
- cystic fibrosis
- red blood cell
- gas chromatography
- silver nanoparticles
- tandem mass spectrometry