Highly multiplexed single-cell RNA-seq by DNA oligonucleotide tagging of cellular proteins.
Jase GehringJong Hwee ParkSisi ChenMatthew ThomsonLior PachterPublished in: Nature biotechnology (2019)
We describe a universal sample multiplexing method for single-cell RNA sequencing in which fixed cells are chemically labeled by attaching identifying DNA oligonucleotides to cellular proteins. Analysis of a 96-plex perturbation experiment revealed changes in cell population structure and transcriptional states that cannot be discerned from bulk measurements, establishing an efficient method for surveying cell populations from large experiments or clinical samples with the depth and resolution of single-cell RNA sequencing.
Keyphrases
- single cell
- rna seq
- high throughput
- single molecule
- circulating tumor
- cell free
- induced apoptosis
- gene expression
- nucleic acid
- cell cycle arrest
- transcription factor
- optical coherence tomography
- computed tomography
- endoplasmic reticulum stress
- cell proliferation
- oxidative stress
- circulating tumor cells
- heat stress
- genetic diversity