Morinda citrifolia leaf assisted synthesis of ZnO decorated Ag bio-nanocomposites for in-vitro cytotoxicity, antimicrobial and anticancer applications.
Gopinath VenkatramanPriyadarshini Sakthi MohanPuteri Shafinaz Abdul-RahmanFaridah SonsudinBarathan MuttiahAbdurahman Hajinur HiradAbdullah A AlarfajShifa WangPublished in: Bioprocess and biosystems engineering (2024)
This study used Morinda citrifolia leaf (MCL) extract to synthesise Zinc oxide nanoparticles (ZnO NPs) and ZnO decorated silver nanocomposites (ZnO/Ag NCs). The synthesized nanomaterials structural morphology and crystallinity were characterized using a Field emission scanning electron microscope (FESEM) and X-ray diffraction (XRD) analysis. The antimicrobial activity of ZnO NPs and ZnO/Ag NCs was evaluated using human nosocomial bacterial pathogens. The highest antimicrobial activity was recorded for ZnO/Ag NCs at the minimum inhibitory concentration (MIC) at 80 and 100 μg/mL for Escherichia coli, Pseudomonas aeruginosa and Bacillus subtilis, Staphylococcus aureus than ZnO NPs at the MIC of 120 and 140 μg/mL for Bacillus subtilis and Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus. Furthermore, ROS detection, viability assay and bacterial membrane integrity analysis of ZnO/Ag NCs treated P. aeruginosa and S. aureus revealed the fundamental bactericidal mechanism involving cell wall, cell membrane interaction and release of cytoplasmic contents. In addition, ZnO/Ag NCs and ZnO NPs showed higher toxicity towards A549 lung cancer cells than the non-cancerous RAW264 macrophage cells, with IC 50 of 242 and 398 µg/mL respectively, compared to IC 50 of 402 and 494 µg/mL for the macrophage cells. These results suggest that the ZnO/Ag NCs can be effectively used to develop antimicrobial and anticancer materials.
Keyphrases
- quantum dots
- visible light
- reduced graphene oxide
- room temperature
- staphylococcus aureus
- oxide nanoparticles
- escherichia coli
- pseudomonas aeruginosa
- sensitive detection
- bacillus subtilis
- gold nanoparticles
- induced apoptosis
- adipose tissue
- biofilm formation
- cell death
- endothelial cells
- light emitting
- oxidative stress
- cystic fibrosis
- signaling pathway
- cell cycle arrest
- cell proliferation
- computed tomography
- pi k akt
- acinetobacter baumannii
- drug resistant