Bioorthogonal double-fluorogenic siliconrhodamine probes for intracellular super-resolution microscopy.
E KozmaGemma Estrada GironaGiulia PaciE A LemkePéter KelePublished in: Chemical communications (Cambridge, England) (2018)
A series of double-fluorogenic siliconrhodamine probes were synthesized. These tetrazine-functionalized, membrane-permeable labels allowed site-specific bioorthogonal tagging of genetically manipulated intracellular proteins and subsequent imaging using super-resolution microscopy.