Harmonizing the growing fluorogenic RNA aptamer toolbox for RNA detection and imaging.

The field of fluorogenic RNA aptamers is a burgeoning research area that aims to address the lack of naturally fluorescent RNA molecules for RNA detection and imaging. These small RNA tags bind to their fluorogenic ligands resulting in significant fluorescent enhancement, leading to a molar brightness comparable to or exceeding that of fluorescent proteins. In the past decade, multiple light-up RNA aptamer systems have been isolated that bind to a broad range of ligands involving several distinct mechanisms of fluorogenicity. This review discusses the selection methods used to isolate fluorogenic RNA aptamers. More than seventy fluorogenic aptamer:ligand pairs are evaluated using objective parameters ( e.g. , molar brightness, binding affinity, fluorophore exchange capabilities and other details). General guidelines for choosing fluorescent RNA tools, with an emphasis on single-molecule detection and multi-colour imaging applications are provided. Lastly the importance of global standards for evaluating fluorogenic RNA aptamer systems is discussed.