3-Benzoylquinoxalinone as a photoaffinity labelling derivative with fluorogenic properties allowing reaction monitoring under "no-wash" conditions.

Madeleine CauwelClément GuillouKévin RenaultDamien SchapmanMagalie BénardLudovic GalasPascal CosettePierre-Yves Renard Cyrille Sabot

Published in: Chemical communications (Cambridge, England) (2021)

Described herein is a quinoxalinone-based photoaffinity probe with caged fluorescence properties. Upon visible blue LED irradiation (λmax 450 nm), this photo-crosslinker is able to covalently capture proteins with concomitant fluorescence labelling. This process enables monitoring applications under "no wash" conditions.

Keyphrases

single molecule
light emitting
energy transfer
living cells
photodynamic therapy
quantum dots
fluorescent probe