Direct Detection of Circulating Tumor Cells in Whole Blood Using Time-Resolved Luminescent Lanthanide Nanoprobes.
Hanhan GuoXiaorong SongWen LeiCheng HeWenwu YouQingzhong LinShanyong ZhouXueyuan ChenZhuo ChenPublished in: Angewandte Chemie (International ed. in English) (2019)
The detection of circulating tumor cells (CTCs) is crucial to early cancer diagnosis and the evaluation of cancer metastasis. However, it remains challenging due to the scarcity of CTCs in the blood. Herein, we report an ultrasensitive platform for the direct detection of CTCs using luminescent lanthanide nanoprobes. These were designed to recognize the epithelial cell adhesion molecules on cancer cells, allowing signal amplification through dissolution-enhanced time-resolved photoluminescence (TRPL) and the elimination of short-lived autofluorescence interference. This enabled the direct detection of blood breast-cancer cells with a limit of detection down to 1 cell/well of a 96-well plate. Moreover, blood CTCs (≥10 cells mL-1 ) can be detected in cancer patients with a detection rate of 93.9 % (14/15 patients). We envision that this ultrasensitive detection platform with excellent practicality may provide an effective strategy for early cancer diagnosis and prognosis evaluation.
Keyphrases
- circulating tumor cells
- label free
- loop mediated isothermal amplification
- papillary thyroid
- real time pcr
- quantum dots
- squamous cell
- stem cells
- circulating tumor
- energy transfer
- young adults
- newly diagnosed
- single molecule
- mesenchymal stem cells
- mass spectrometry
- cell therapy
- metal organic framework
- chronic kidney disease
- fluorescence imaging
- molecularly imprinted
- simultaneous determination
- peritoneal dialysis
- patient reported