A Bioluminescent Biosensor for Quantifying the Interaction of SARS-CoV-2 and Its Receptor ACE2 in Cells and In Vitro.
Xiaolong YangLidong LiuYawei HaoEva SoSahar Sarmasti EmamiDerek ZhangYanping GongPrameet M ShethYutian WangPublished in: Viruses (2021)
The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is currently spreading and mutating with increasing speed worldwide. Therefore, there is an urgent need for a simple, sensitive, and high-throughput (HTP) assay to quantify virus-host interactions in order to quickly evaluate the infectious ability of mutant viruses and to develop or validate virus-inhibiting drugs. Here, we developed an ultrasensitive bioluminescent biosensor to evaluate virus-cell interactions by quantifying the interaction between the SARS-CoV-2 receptor binding domain (RBD) and its cellular receptor angiotensin-converting enzyme 2 (ACE2) both in living cells and in vitro. We have successfully used this novel biosensor to analyze SARS-CoV-2 RBD mutants and evaluated candidate small molecules (SMs), antibodies, and peptides that may block RBD:ACE2 interaction. This simple, rapid, and HTP biosensor tool will significantly expedite the detection of viral mutants and the anti-COVID-19 drug discovery process.
Keyphrases
- sars cov
- angiotensin converting enzyme
- respiratory syndrome coronavirus
- label free
- angiotensin ii
- gold nanoparticles
- high throughput
- sensitive detection
- quantum dots
- living cells
- drug discovery
- loop mediated isothermal amplification
- fluorescent probe
- single cell
- induced apoptosis
- wild type
- coronavirus disease
- single molecule
- signaling pathway
- oxidative stress
- cell therapy
- cell cycle arrest