Psychiatric-disorder-related behavioral phenotypes and cortical hyperactivity in a mouse model of 3q29 deletion syndrome.
Masayuki BabaKazumasa YokoyamaKaoru SeirikiYuichiro NakaKensuke MatsumuraMomoka KondoKana YamamotoMisuzu HayashidaAtsushi KasaiYukio AgoKazuki NagayasuAtsuko Hayata-TakanoAkinori TakahashiShun YamaguchiDaisuke MoriNorio OzakiTadashi YamamotoKazuhiro TakumaRyota HashimotoHitoshi HashimotoTakanobu NakazawaPublished in: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology (2019)
3q29 microdeletion, a rare recurrent copy number variant (CNV), greatly confers an increased risk of psychiatric disorders, such as schizophrenia and autism spectrum disorder (ASD), as well as intellectual disability. However, disease-relevant cellular phenotypes of 3q29 deletion syndrome remain to be identified. To reveal the molecular and cellular etiology of 3q29 deletion syndrome, we generated a mouse model of human 3q29 deletion syndrome by chromosome engineering, which achieved construct validity. 3q29 deletion (Df/+) mice showed reduced body weight and brain volume and, more importantly, impaired social interaction and prepulse inhibition. Importantly, the schizophrenia-related impaired prepulse inhibition was reversed by administration of antipsychotics. These findings are reminiscent of the growth defects and neuropsychiatric behavioral phenotypes in patients with 3q29 deletion syndrome and exemplify that the mouse model achieves some part of face validity and predictive validity. Unbiased whole-brain imaging revealed that neuronal hyperactivation after a behavioral task was strikingly exaggerated in a restricted region of the cortex of Df/+ mice. We further elucidated the cellular phenotypes of neuronal hyperactivation and the reduction of parvalbumin expression in the cortex of Df/+ mice. Thus, the 3q29 mouse model provides invaluable insight into the disease-causative molecular and cellular pathology of psychiatric disorders.
Keyphrases
- mouse model
- autism spectrum disorder
- intellectual disability
- copy number
- case report
- body weight
- mitochondrial dna
- bipolar disorder
- genome wide
- endothelial cells
- cerebral ischemia
- white matter
- dna methylation
- gene expression
- multiple sclerosis
- single cell
- type diabetes
- metabolic syndrome
- brain injury
- photodynamic therapy
- adipose tissue
- long non coding rna
- subarachnoid hemorrhage
- fluorescence imaging
- mass spectrometry