A novel highly fluorescent S, N, O co-doped carbon dots for biosensing and bioimaging of copper ions in live cells.
Yanyu DaiZhichao LiuYunfeng BaiZezhong ChenJun QinFeng FengPublished in: RSC advances (2018)
In this study, novel highly fluorescent sulfur, nitrogen, and oxygen co-doped carbon dots (S, N, O-CDs) were prepared from m -phenylenediamine and sulfamide by using the hydrothermal method. The prepared S, N, O-CDs show high doping rate and fluorescence yield as well as long-term fluorescence stability. In addition, S, N, O-CDs show good fluorescence response towards Cu 2+ over a concentration range of 2-60 μM with a detection limit of 0.29 μM. Taking advantages of the properties of S, N, O-CDs including high selectivity and low cytotoxicity, S, N, O-CDs were successfully applied for Cu 2+ sensing and imaging in the cells and O 2 ˙ - -induced Cu 2+ increase in the cells was observed. Furthermore, on account of strong complexation between Cu 2+ and pyrophosphate ion (PPi) as well as specific hydrolysis ability of alkaline phosphatase (ALP) towards PPi, PPi and ALP were further detected with high selectivity based on S, N, O-CDs/Cu 2+ system. The prepared S, N, O-CDs showed good detection results for PPi and ALP with detection limits of 0.44 μM and 1.03 U L -1 , respectively. Moreover, the developed method also realized PPi and ALP detection in real samples.
Keyphrases
- quantum dots
- energy transfer
- sensitive detection
- induced apoptosis
- loop mediated isothermal amplification
- cell cycle arrest
- label free
- protein protein
- real time pcr
- aqueous solution
- single molecule
- visible light
- signaling pathway
- endoplasmic reticulum stress
- metal organic framework
- oxidative stress
- high resolution
- small molecule
- cell proliferation
- pi k akt
- high glucose
- heavy metals
- drug induced
- living cells