From a Laboratory Exercise for Students to a Pioneering Biosensing Technology.

Surface plasmon resonance (SPR) for biosensing was demonstrated 30 years ago. In the present contribution, its general background is described together with the necessary developments both in instrumentation and surface chemistry, leading to the final so-called BIAcore technology. The description is naturally colored by my personal opinion of the developments. SPR for the elucidation of organic mono- and multilayers introduced at the end of the 1970s formed the basis for the first biosensing demonstration of SPR in the beginning of the 1980s. It is pointed out how the need of an up-to-date laboratory exercise for the undergraduate students and the multidisciplinary environment at the Laboratory of Applied Physics at Linköping University led to this demonstration. The initial experiments are touched upon and the further developments at Pharmacia, which led to the BIAcore technology, are described in some details. Some of the present activities in Linköping related to optical biosensing with ubiquitous instrumentation are also described, including SPR detection with a computer screen and a web camera and most recently with a cellular phone.