Single-cell analyses to reveal hematopoietic stem cell fate decisions.
Ilaria LungerMalak FawazMichael A RiegerPublished in: FEBS letters (2017)
Hematopoietic stem cells (HSCs) are the best studied adult stem cells with enormous clinical value. Most of our knowledge about their biology relies on assays at the single HSC level. However, only the recent advances in developing new single cell technologies allowed the elucidation of the complex regulation of HSC fate decision control. This Review will focus on current attempts to investigate individual HSCs at molecular and functional levels. The advantages of these technologies leading to groundbreaking insights into hematopoiesis will be highlighted, and the challenges facing these technologies will be discussed. The importance of combining molecular and functional assays to enlighten regulatory networks of HSC fate decision control, ideally at high temporal resolution, becomes apparent for future studies.
Keyphrases
- single cell
- hematopoietic stem cell
- high throughput
- stem cells
- rna seq
- induced apoptosis
- single molecule
- healthcare
- decision making
- cell cycle arrest
- signaling pathway
- transcription factor
- current status
- oxidative stress
- cell death
- young adults
- cell therapy
- gene expression
- bone marrow
- computed tomography
- dna methylation
- case control
- diffusion weighted imaging