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In vivo bioluminescence imaging of the spatial and temporal colonization of lactobacillus plantarum 423 and enterococcus mundtii ST4SA in the intestinal tract of mice.

Winschau F Van ZylShelly M DeaneLeon Milner Theodore Dicks
Published in: BMC microbiology (2018)
Our findings demonstrate the efficiency of a high-copy number vector, constitutive promoters and bioluminescence imaging to study the colonization and persistence of L. plantarum 423 and E. mundtii ST4SA in the murine GIT. The system allowed us to differentiate between intestinal transit times of the two strains in the digestive tract. This is the first report of bioluminescence imaging of a luciferase-expressing E. mundtii strain to study colonization dynamics in the murine model. The bioluminescence system developed in this study may be used to study the in vivo colonization dynamics of other probiotic LAB.
Keyphrases
  • copy number
  • escherichia coli
  • type diabetes
  • adipose tissue
  • pseudomonas aeruginosa
  • mass spectrometry