Insights into the time-course cellular effects triggered by iron oxide nanoparticles by combining proteomics with the traditional pharmacology strategy.
Yuqing MengYanqing LiuYongping ZhuChong QiuAng MaDandan LiuShujie ZhangLiwei GuJunzhe ZhangJi-Gang WangPublished in: Journal of materials chemistry. B (2024)
In recent years, a number of initially approved magnetic iron oxide nanoparticle (IONP)-based nano-medicines have been withdrawn due to the obscure nano-bio effects. Therefore, there is an urgent need to study the cellular effects triggered by IONPs on cells. In this study, we investigate the time-course cellular effects on the response of RAW 264.7 cells caused by Si-IONPs via pharmacological and mass spectrometry-based proteomics techniques. Our results revealed that Si-IONPs were internalized by clathrin-mediated endocytosis within 1 hour, and gradually degraded in endolysosomes over time, which might influence autophagy, oxidative stress, innate immune response, and inflammatory response after 12 hours. Our research provides a necessary assessment of Si-IONPs for further clinical treatment.
Keyphrases
- mass spectrometry
- immune response
- induced apoptosis
- oxidative stress
- inflammatory response
- iron oxide
- cell cycle arrest
- endoplasmic reticulum stress
- cell death
- blood pressure
- signaling pathway
- room temperature
- dna damage
- liquid chromatography
- toll like receptor
- single cell
- dendritic cells
- lipopolysaccharide induced
- solid phase extraction
- replacement therapy