DNA variants detected in primary and metastatic lung adenocarcinoma: a case report and review of the literature.
Christina KellyCaitlin RaymondSong HanYoumin LinLinyijia ChenGengming HuangJianli DongPublished in: Laboratory medicine (2024)
Non-small cell lung cancer (NSCLC) has been found to have recurrent genetic abnormalities, and novel therapies targeting these aberrations have improved patient survival. In this study, specimens from benign tissue, primary tumors, and brain metastases were obtained at autopsy from a 55-year-old White female patient diagnosed with NSCLC and were examined using next-generation sequencing (NGS) and chromosomal microarray assay (CMA). No genetic aberrations were noted in the benign tissue; however, NGS identified a mutation in the KRAS proto-oncogene, GTPase (KRAS): KRAS exon 2 p.G12D in primary and metastatic tumor specimens. We observed 7 DNA copy number aberrations (CNAs) in primary and metastatic tumor specimens; an additional 7 CNAs were exclusively detected in the metastatic tumor specimens. These DNA alterations may be genetic drivers in the pathogenesis of the tumor specimen from our patient and may serve as biomarkers for the classification and prognosis of NSCLC.
Keyphrases
- copy number
- small cell lung cancer
- mitochondrial dna
- brain metastases
- genome wide
- squamous cell carcinoma
- circulating tumor
- dna methylation
- case report
- cell free
- single molecule
- advanced non small cell lung cancer
- deep learning
- machine learning
- wild type
- high throughput
- fine needle aspiration
- gene expression
- free survival
- cancer therapy
- drug delivery
- tyrosine kinase
- bioinformatics analysis