Spatial and temporal niche separation of methanomassiliicoccales phylotypes in temperate fens.

The hydrogen-dependent and methylotrophic order Methanomassiliicoccales consists of the families Methanomethylophilaceae and Methanomassiliicoccaceae. While Methanomethylophilaceae are comparatively well studied, there is a lack of knowledge on Methanomassiliicoccaceae. In this 16S rRNA gene amplicon sequencing based study we investigated the temporal and spatial dynamics of the Methanomassiliicoccales in drained and rewetted sites of three common temperate fen peatlands. A 2.5-year-monitoring of the fen microbiome composition in three peat depths revealed a dynamic methanogen and Methanomassiliicoccales composition across space and time. Four Methanomassiliicoccales phylotypes were found and they were differentially distributed between the fen types. The wetland cluster phylotype was omnipresent and dominant in abundance in all sites along all depths. The Methanomassiliicoccales phylotype was highly abundant in topsoil while the AB364942 phylotype was exclusively found in deeper regions of the rewetted percolation fen. The phylotype affiliated with Methanomassiliicoccales strain U3.2.1 was only detected in the rewetted percolation fen. We discussed the distribution of the four phylotypes with implications to their ecophysiology, where oxygen tolerance and substrate spectrum might play major roles. In conclusion, the Methanomassiliicoccales are widespread and do account for a significant proportion of methanogens, which might suggest their importance for methane emissions from peatlands.