A Photoenzymatic NADH Regeneration System.

Georg T HöflerElena Fernández-FueyoMilja PesicSabry H YounesEun-Gyu ChoiYong H KimVlada B UrlacherIsabel W C E ArendsFrank Hollmann

Published in: Chembiochem : a European journal of chemical biology (2018)

A photoenzymatic NADH regeneration system was established. The combination of deazariboflavin as a photocatalyst with putidaredoxin reductase enabled the selective reduction of NAD+ into the enzyme-active 1,4-NADH to promote an alcohol dehydrogenase catalysed stereospecific reduction reaction. The catalytic turnover of all the reaction components was demonstrated. Factors influencing the efficiency of the overall system were identified.

Keyphrases

stem cells
wound healing
bone mineral density
electron transfer
visible light
highly efficient
postmenopausal women