Inhibition of growth and gene expression in Staphylococcus aureus by anti-gyrA peptide nucleic acid.
Mahnaz TavakoliAli HashemiMaryam VaezjalaliMohammad MohammadzadehMohammad MohammadzadehPublished in: Future microbiology (2020)
Aim: Multidrug-resistant Staphylococcus aureus isolates have become a serious concern in clinical microbiology. Antisense strategy, which specifically targets essential genes, could be helpful. Materials & methods: S. aureus cultures were treated with peptide conjugate-peptide nucleic acid (PPNA) specific for the gyrA gene. In addition, antimicrobial synergy with ciprofloxacin was tested. Results: The results indicated anti-gyrA-PPNA dramatically inhibited the growth of S. aureus isolates in Mueller Hinton Broth with complete elimination of bacteria observed on cell cultures. Specifically, PPNA reduced the gyrA transcripts up to 50%. With antisense interference, growth inhibition was augmented through combination with ciprofloxacin. Conclusion: This study suggested that anti-gyrA-PPNAs could be introduced as a novel candidate for developing antisense antibiotic to treat all S. aureus infections.
Keyphrases
- nucleic acid
- staphylococcus aureus
- gene expression
- multidrug resistant
- genome wide
- biofilm formation
- dna methylation
- single cell
- cell therapy
- stem cells
- methicillin resistant staphylococcus aureus
- genome wide identification
- mesenchymal stem cells
- cystic fibrosis
- klebsiella pneumoniae
- virtual reality
- infectious diseases