[Histone-like protein H-NS as a negative regulator of quorum sensing systems in gram-negative bacteria].
O E MelkinaI I GoryaninG B ZavilgelskyPublished in: Genetika (2018)
The effects of histone-like protein H-NS on transcription of promoters of the Quorum Sensing regulated operons from marine luminescent mesophilic bacterium Aliivibrio fischeri and psychrophilic Aliivibrio logei, as well as from pathogenic Pseudomonas aeruginosa, are studied. In the present work, the plasmids carrying DNA fragments with the promoters Pr1f (upstream of the luxICDABEG operon from A. fischeri), Pr1l (upstream of the luxCDABEG operon from A. logei), Pr2l (upstream of luxI gene from A. logei), PluxCf (upstream of luxC gene from A. fischeri), and PlasI (upstream of lasI gene from P. aerugenosa) are used. In these plasmids, promoter-operator regions are transcriptionally fused to the reporter genes cassette luxCDABE from Photorhabdus luminescens. Here we have shown that the transcription of the QS-regulated promoters in E. coli hns::kan cells increases 100 to 1000 times. Furthermore, transcription of the QS-regulated promoters in E. coli hns + cells increases 10 to 100 times in the cells transformed with the plasmid carrying gene ardA ColIb-P9 encoding DNA mimic antirestriction protein ArdA, inhibitor of the type I restriction-modification systems.
Keyphrases
- transcription factor
- escherichia coli
- induced apoptosis
- genome wide identification
- genome wide
- pseudomonas aeruginosa
- cell cycle arrest
- dna methylation
- copy number
- endoplasmic reticulum stress
- crispr cas
- circulating tumor
- cystic fibrosis
- cell proliferation
- gene expression
- quantum dots
- cell death
- dengue virus
- staphylococcus aureus
- drug resistant
- zika virus
- multidrug resistant
- candida albicans
- pi k akt
- amino acid