ssDNA is not superior to dsDNA as long HDR donors for CRISPR-mediated endogenous gene tagging in human diploid RPE1 and HCT116 cells.

Akira MabuchiShoji HataMariya GenovaChiharu TeiKei K ItoMasayasu HirotaTakuma KomoriMasamitsu FukuyamaTakumi ChinenAtsushi ToyodaDaiju Kitagawa

Published in: BMC genomics (2023)

These results indicate that ssDNA is not superior to dsDNA as long HDR donors with relatively short homology arms for gene knock-in in human RPE1 and HCT116 cells.

Keyphrases

cell cycle arrest
induced apoptosis
endothelial cells
genome wide
cell death
induced pluripotent stem cells
copy number
endoplasmic reticulum stress
crispr cas
genome wide identification
gene expression
kidney transplantation
transcription factor
genome wide analysis