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Capillary-Mediated Single-Cell Dispenser.

Li WuShihan XuJingang WangAmy L PaguiriganJerald P RadichYuling QinDaniel T Chiu
Published in: Analytical chemistry (2021)
Single-cell manipulation, sorting, and dispensing into multiwell plates is useful for single-cell multiomics studies. Here, we develop a single-cell dispenser inspired by electrohydrodynamic jet printing that achieves accurate droplet generation and single-cell sorting and dispensing using fused silica capillary tubing as both the optical detection window and nozzle for droplet dispensing. Parameters that affect droplet dispensing performance-capillary inner and outer diameter, flow rate, applied voltage, and solution properties-were optimized systematically with COMSOL simulations and experimentation. Small (5-10 nL) droplets were obtained by using 100-μm inner diameter and 160-μm outer diameter capillary tubing and allowed efficient encapsulation and dispensing of single cells. We demonstrate an application of this easy-to-assemble single-cell dispenser by sorting and dispensing cells into multiwell plates for single-cell PCR analysis.
Keyphrases
  • single cell
  • rna seq
  • high throughput
  • induced apoptosis
  • high resolution
  • cell cycle arrest
  • mass spectrometry
  • endoplasmic reticulum stress
  • cell death
  • data analysis
  • real time pcr