A rapid virus-induced gene silencing (VIGS) method for assessing resistance and susceptibility to cassava mosaic disease.

Getu BeyeneRaj Deepika ChauhanNigel J Taylor

Published in: Virology journal (2017)

A rapid VIGS-based screening system was developed for assessing resistance and susceptibility to CMD. The method is space and resource efficient, reducing the time required to perform CMD screening to as little as 2-4 weeks. It can be employed as a high throughput rapid screening system to assess new cassava cultivars and for screening transgenic, gene edited and breeding lines under controlled growth conditions.

Keyphrases

high throughput
loop mediated isothermal amplification
gene expression
oxidative stress
single cell
gestational age