Denervated hippocampus provides a favorable microenvironment for neuronal differentiation of endogenous neural stem cells.
Lei ZhangXiao HanXiang ChengXue-Feng TanHe-Yan ZhaoXin-Hua ZhangPublished in: Neural regeneration research (2016)
Fimbria-fornix transection induces both exogenous and endogenous neural stem cells to differentiate into neurons in the hippocampus. This indicates that the denervated hippocampus provides an environment for neuronal differentiation of neural stem cells. However, the pathways and mechanisms in this process are still unclear. Seven days after fimbria fornix transection, our reverse transcription polymerase chain reaction, western blot assay, and enzyme linked immunosorbent assay results show a significant increase in ciliary neurotrophic factor mRNA and protein expression in the denervated hippocampus. Moreover, neural stem cells derived from hippocampi of fetal (embryonic day 17) Sprague-Dawley rats were treated with ciliary neurotrophic factor for 7 days, with an increased number of microtubule associated protein-2-positive cells and decreased number of glial fibrillary acidic protein-positive cells detected. Our results show that ciliary neurotrophic factor expression is up-regulated in the denervated hippocampus, which may promote neuronal differentiation of neural stem cells in the denervated hippocampus.
Keyphrases
- neural stem cells
- cerebral ischemia
- prefrontal cortex
- induced apoptosis
- cognitive impairment
- subarachnoid hemorrhage
- cell cycle arrest
- blood brain barrier
- high throughput
- binding protein
- stem cells
- spinal cord
- endoplasmic reticulum stress
- spinal cord injury
- neuropathic pain
- long non coding rna
- ionic liquid
- single cell
- pi k akt