Mapping DNA methylation with high-throughput nanopore sequencing.

Arthur C RandMiten JainJordan M EizengaAudrey Musselman-BrownHugh E OlsenMark AkesonBenedict Paten

Published in: Nature methods (2017)

DNA chemical modifications regulate genomic function. We present a framework for mapping cytosine and adenosine methylation with the Oxford Nanopore Technologies MinION using this nanopore sequencer's ionic current signal. We map three cytosine variants and two adenine variants. The results show that our model is sensitive enough to detect changes in genomic DNA methylation levels as a function of growth phase in Escherichia coli.

Keyphrases

dna methylation
copy number
single molecule
genome wide
solid state
high throughput
escherichia coli
high density
gene expression
high resolution
single cell
mass spectrometry
cystic fibrosis
klebsiella pneumoniae
biofilm formation
staphylococcus aureus