Development and validation of a LC-PDA method for methylphenidate analysis in sewage.
Maiara C S PaixãoJessica NardiCharise D BertolNatália FreddoBruna F VieiraVitoria A RosanoMaria T FriedrichLuciana Grazziotin Rossato-GrandoPublished in: Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering (2021)
Methylphenidate (MPH) is an important emerging pollutant found in effluents and wastewater. Thus, we aimed to develop and validate a method for detection and quantitation of MPH residues in sewage through high performance liquid chromatography coupled with photodiode array detector (LC-PDA). Here we describe a selective, accurate, precise, and valid method for determination of MPH in sewage with a total running time of 10 min, with limits of detection and quantification of 0.27 and 0.92 µg/mL, respectively. MPH retention peak was observed at 5 min. The method was applied to MPH analysis in a sewage sample pretreated with solid phase extraction, obtaining a result of 2.8 µg/L of MPH. Thus, the developed method can be considered feasible to be applied to MPH residual contamination analysis in sewage using a widely available apparatus.
Keyphrases
- solid phase extraction
- high performance liquid chromatography
- simultaneous determination
- tandem mass spectrometry
- liquid chromatography tandem mass spectrometry
- molecularly imprinted
- mass spectrometry
- liquid chromatography
- gas chromatography mass spectrometry
- ultra high performance liquid chromatography
- wastewater treatment
- antibiotic resistance genes
- high resolution
- gas chromatography
- high throughput
- loop mediated isothermal amplification
- magnetic resonance
- high intensity
- working memory
- single cell
- high density
- quantum dots