A chemosensor for micro- to nano-molar detection of Ag+ and Hg2+ ions in pure aqueous media and its applications in cell imaging.
Jitendra P NandreSamadhan R PatilSuban Kumar SahooChullikkattil P PradeepAndrei ChurakovFabiao YuLingxin ChenCarl RedshawAshok A PatilUmesh D PatilPublished in: Dalton transactions (Cambridge, England : 2003) (2018)
The pyridine substituted thiourea derivative PTB-1 was synthesized and characterized by spectroscopic techniques as well as by single crystal X-ray crystallography. The metal ion sensing ability of PTB-1 was explored by various experimental (naked-eye, UV-Vis, fluorescence, mass spectrometry and 1H NMR spectroscopy) and theoretical (B3LYP/6-31G**/LANL2DZ) methods. PTB-1 exhibited a highly selective naked-eye detectable color change from colorless to dark brown and UV-Vis spectral changes for the detection of Ag+ with a detection limit of 3.67 μM in aqueous medium. The detection of Ag+ ions was achieved by test paper strip and supported silica methods. In contrast, PTB-1 exhibited a 23-fold enhanced emission at 420 nm in the presence of Hg2+ ions with a nano-molar detection limit of 0.69 nM. Finally, the sensor PTB-1 was applied successfully for the intracellular detection of Hg2+ ions in a HepG2 liver cell line, which was monitored by the use of confocal imaging techniques.
Keyphrases
- aqueous solution
- quantum dots
- loop mediated isothermal amplification
- real time pcr
- high resolution
- mass spectrometry
- magnetic resonance
- magnetic resonance imaging
- optical coherence tomography
- molecular docking
- computed tomography
- mesenchymal stem cells
- single cell
- highly efficient
- bone marrow
- single molecule
- capillary electrophoresis
- visible light