Large-Scale Production of LGR5-Positive Bipotential Human Liver Stem Cells.
Kerstin SchneebergerNatalia Sánchez-RomeroShicheng YeFrank G van SteenbeekLoes A OosterhoffIris Pla PalacinChen ChenMonique E van WolferenGilles van TienderenRuby LieshoutHaaike Colemonts-VroninksImre ScheneRuurdtje HoekstraMonique M A VerstegenLuc J W van der LaanLouis C PenningSabine A FuchsHans CleversJoery De KockPedro M BaptistaBart SpeePublished in: Hepatology (Baltimore, Md.) (2020)
We established a highly efficient method for culturing large numbers of LGR5-positive stem cells in the form of organoids, which paves the way for the application of organoids for tissue engineering and liver transplantation.