The Development of Cytogenetic Maps for Malaria Mosquitoes.
Gleb N ArtemovVladimir N StegniyMaria V SharakhovaIgor V SharakhovPublished in: Insects (2018)
Anopheline mosquitoes are important vectors of human malaria. Next-generation sequencing opens new opportunities for studies of mosquito genomes to uncover the genetic basis of a Plasmodium transmission. Physical mapping of genome sequences to polytene chromosomes significantly improves reference assemblies. High-resolution cytogenetic maps are essential for anchoring genome sequences to chromosomes as well as for studying breakpoints of chromosome rearrangements and chromatin protein localization. Here we describe a detailed pipeline for the development of high-resolution cytogenetic maps using polytene chromosomes of malaria mosquitoes. We apply this workflow to the refinement of the cytogenetic map developed for Anopheles beklemishevi.
Keyphrases
- aedes aegypti
- high resolution
- plasmodium falciparum
- dengue virus
- zika virus
- genome wide
- copy number
- endothelial cells
- mass spectrometry
- dna damage
- gene expression
- mental health
- transcription factor
- physical activity
- dna methylation
- tandem mass spectrometry
- oxidative stress
- high speed
- small molecule
- genetic diversity
- binding protein