Establishment of primary human breast cancer cell lines using "pulsed hypoxia" method and development of metastatic tumor model in immunodeficient mice.
Anna A NushtaevaAnastasia A KarpushinaMikhail S ErmakovLudmila F GulyaevaAlexey V GerasimovSergey V SidorovTatyana A GaynerAnastasia Y YunusovaAnastasia V TkachenkoVladimir A RichterOlga A KovalPublished in: Cancer cell international (2019)
The developed BC cells metastasizing to mediastinum lymph nodes are a relevant model for downstream applications. Moreover, our findings demonstrate that pulsed hypoxia induces transformation of primary fibroblastoid breast cancer cells to epithelial-like cells and both of these cultures-induced and original-don't show tumor initiating capacity.
Keyphrases
- endothelial cells
- lymph node
- high glucose
- breast cancer cells
- induced apoptosis
- squamous cell carcinoma
- small cell lung cancer
- cell cycle arrest
- diabetic rats
- induced pluripotent stem cells
- adipose tissue
- high fat diet induced
- skeletal muscle
- signaling pathway
- cell proliferation
- young adults
- early stage
- insulin resistance
- wild type